Screen of Pinus massoniana for Resistance to Pinewood Nematode: In Vitro Propagation and Evaluation of Regenerated Microshoots

To accelerate breeding of Pinus massoniana Lamb. resistance to pine wilt disease (PWD), caused by the pinewood nematode (PWN), Bursaphelenchus xylophilus, a protocol was established for in vitro propagation P. and evaluation regenerated microshoots from different clones PWN. Axillary bud induction achieved culturing cotyledonary node explants 3-week-old seedlings Gupta Durzan (DCR) medium that supplemented with 4 mg L−1 6-benzyladenine (BA) 0.2 α-naphthaleneacetic acid (NAA). Explants induced buds were transferred DCR without plant growth regulator facilitate elongation. Stem segments elongated shoots used as propagules further shoot multiplication. Six-month-old met requirements test inoculated aseptic PWN (500 PWNs/shoot). The wilting rate varied between 20% 100%, 18 days after inoculation. Except Clone 227, which showed highest 0%, other various degrees 30 d number nematodes recovered 227 significantly lower than clones. This study promotes provides an effective method host/pathogen interaction massoniana.